DNA Release Dynamics from Reducible Polyplexes by Atomic Force Microscopy
نویسندگان
چکیده
منابع مشابه
DNA release dynamics from reducible polyplexes by atomic force microscopy.
Controlled intracellular disassembly of polyelectrolyte complexes of polycations and DNA (polyplexes) is a crucial step for the success of nonviral gene delivery. Motivated by our previous observation of different gene delivery performances among multiblock reducible copolypeptide vectors ( Manickam, D. S. ; Oupicky, D. Bioconjugate Chem. 2006, 17, 1395- 1403 ), atomic force microscopy is used ...
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W a) " NA sizing is one of the most widely used analytical methods in molecular _6 42 biology and biochemistry, and it is a core method for genomic analysis. Many research, • toE health care, and forensic applications rely on DNA sizing. Given the volume of DNA C: sizing done and the associated cost, an increasing need exists for extremely rapid, high. throughput, and inexpensive techniques. Es...
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The DNA release dynamics of bioreducible poly(amido amine) polyplexes were studied in real time by atomic force microscopy (AFM). DNA release is triggered by a depolymerization of high-molecular-weight polycations into low-molecular-weight oligocations that occurs by means of the thiol and disulfide exchange reaction mechanism. AFM images were captured in a simulated physiological reducing envi...
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This chapter reviews recent advances in the understanding of DNA release dynamics from polymeric gene delivery systems. Atomic force microscopy (AFM) has recently been applied to monitor DNA release from bioreducible polyplex nanoparticles and layer-by-layer (LbL) thin films in real time. DNA release dynamics is central to the understanding and control of gene delivery from gene delivery nano-s...
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We have constructed an atomic force microscope enabling one to image the topography of a sample, and to monitor simultaneously ultrasonic surface vibrations in the MHz range. For detection of the distribution of the ultrasonic vibration amplitude, a part of the position-sensing light beam reflected from the cantilever is directed to an external knife-edge detector. Acoustic images taken on the ...
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ژورنال
عنوان ژورنال: Langmuir
سال: 2008
ISSN: 0743-7463,1520-5827
DOI: 10.1021/la802088y